qtl mapping for seed germination parameters in sunflower (helianthus annuus l.) recombinant inbred lines under salt stress

نویسندگان

آسا آبراهیمی

استادیار، دانشکدة کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات امیرحسین فرتاش

کارشناس ارشد بیوتکنولوژی، دانشکدة کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات علی مفاخری

کارشناس ارشد بیوتکنولوژی، دانشکدة کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات زهرا ساعتیان

کارشناس ارشد بیوتکنولوژی، دانشکدة کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات

چکیده

in order to assess the genetic variability for seed germination parameters under salt stress in sunflower and corresponding qtl locations, an experiment was conducted using a population of 80 recombinant inbred lines obtained from a cross between pac2 and rha266. the experiment was arranged under a randomized complete block design with three replications in control and saline conditions. several germination parameters were measured including time to start germination (tsg), time to 50% germination (t50%g), time to maximum germination (tmg) and percentage of seed germination (psg). results showed highly significant variations among genotypes (rils) for all traits. salt stress had a negative effect on germination, however the significance of ril × condition interaction suggested that the rils have different responses to salt stress. in this study, “1.t50%g.16.1” and “2.t50%g.17.1” qtls (controlling t50%g under salt and control conditions, respectively) were introduced as large-effect qtls. moreover, “2.tsg.16.1” and “1.tsg.16.1” qtls (controlling tsg) and also “2.psg.12.1”, “1.psg.12.1”, “2.psg.12.3” and “1.psg.12.2” qtls (controlling psg) were introduced as stable qtls.

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